p19 mouse embryonal carcinoma cells  (ATCC)

Journal: bioRxiv

Article Title: HENMT1 restricts endogenous retrovirus activity by methylation of 3’-tRNA fragments

doi: 10.1101/2025.05.12.650695

Figure Lengend Snippet: (A) Diagram of cellular tRF3a (∼18 nt) and tRF3b (22 nt) small RNAs derived from mature tRNAs including their post-transcriptional CCA-tail. (B) Work flow of hydrolysis-based tRNA sequencing and small RNA sequencing from the same samples. (C) Quantification of full-length tRNAs, tRF3b and tRF3a fragments. Proportion of tRNA, tRF3a, and tRF3b by amino acid isotype, two biological replicates each, in P19 mouse embryonal carcinoma cells. (D) Relative counts (%) of tRF3a and of tRNA reads that end in CCA. Enrichment of specific isoacceptors is conserved across mouse and human cell lines. mTSC: mouse trophoblast stem cells. (E) Lysine isoacceptor ratios of tRNAs and tRFs in P19 cells. (F) Heat map of log2-fold changes (log2FC) of tRF3a and tRF3b relative to their corresponding full-length tRNA. Asterisks (*) mark significant enrichment (log2FC >1, padj. < 0.01).

Article Snippet: P19 mouse embryonic teratocarcinoma cells (ATCC, #CRL-1825) were grown in Alpha-MEM (Thermo Fisher Scientific, #12571089) with 7.5% bovine calf serum and 2.5% fetal bovine serum (FBS).

Techniques: Derivative Assay, Sequencing, RNA Sequencing

Journal: bioRxiv

Article Title: HENMT1 restricts endogenous retrovirus activity by methylation of 3’-tRNA fragments

doi: 10.1101/2025.05.12.650695

Figure Lengend Snippet: (A) Optimization of alkaline hydrolysis conditions for tRNA hydrolysis sequencing (B) Relates to : Quantification of full-length tRNAs, tRF3b and tRF3a fragments. Proportion of tRNA, tRF-3a, and tRF-3b by amino acid isotype in HeLa cells (two biological replicates) and mTSC (four biological replicates). (C) Proportion of tRNA, tRF-3a, and tRF-3b isoacceptor sequences in all three cell lines (P19, mTSC, HeLa) and their biological replicates. (D) tRNA, tRF-3a, and tRF-3b expression in percent of total reads clustered by similarity for all cell lines. (E) Relates to : Heat map of log2-fold changes (log2FC) of tRF3a and tRF3b relative to their corresponding full-length tRNAs for all isodecoder sequences. Asterisks (*) mark significant enrichment (log2FC >1, adjusted p-value < 0.01). (F) Proportion of Lysine isodecoder tRNA, tRF-3a, and tRF-3b sequences for all three cell lines (P19, mTSC, HeLa). Lys-TTT-3 (yellow) also referred to as Lys 3 is the primer tRNA for MusD/ETn. (G) Phylogenetic tree of human Lysine tRNA isodecoder sequences and Clustal Omega alignment of the 3’-end of cytoplasmic isodecoder sequences. (H) Phylogenetic tree of mouse Lysine tRNA isodecoder sequences and Clustal Omega alignment of the 3’-end of cytoplasmic isodecoder sequences.

Article Snippet: P19 mouse embryonic teratocarcinoma cells (ATCC, #CRL-1825) were grown in Alpha-MEM (Thermo Fisher Scientific, #12571089) with 7.5% bovine calf serum and 2.5% fetal bovine serum (FBS).

Techniques: Sequencing, Expressing

Journal: Neurotrauma Reports

Article Title: A Small-Molecule TrkB/TrkC Ligand Promotes Neurogenesis and Behavioral Recovery Following Traumatic Brain Injury

doi: 10.1089/neur.2024.0117

Figure Lengend Snippet: Neurotrophic agents promote neural progenitor cell proliferation and survival in vitro . (A) Representative images of adult hippocampal progenitor (AHP) and embryonic neural stem (ENS) cells in culture media (CM) containing 0.1% FBS in the presence of the 0.7 nM BDNF or 1 µM LM22B-10. Cultures were maintained for 48 h prior to staining for BrdU; quantitation of BrdU+ cells. (B) AHP cells; LM22B-10 and BDNF increased BrdU+ cells, p < 0.0001 vs. CM, n = 20–40. (C) ENS cells; LM22B-10 increased BrdU+ cells, p < 0.0076 vs. CM, n = 10–20, while changes with BDNF were not significant. (D) P19 cell counts; LM22B-10 at 0.5, 1, and 2 µM similarly increased cell numbers, p = 0.0028, 0.0001, and 0.0002 vs. CM, n = 20. BDNF increased cell numbers, p = 0.0001 vs. CM, n = 20, to a greater extent than observed in 1 µM LM22B-10-exposed cells, p = 0.0001, n = 20 for the number of samples and using Student’s t test. In these studies, n represents sample numbers, the statistical significance of individual comparisons utilizes Student’s t test unless otherwise stated, and scale bars represent 10 µm. BrdU, 5′-bromo-2′-deoxyuridine.

Article Snippet: P19 mouse embryonic carcinoma cells, used for dose–response studies, were purchased from the American Type Culture Collection (ATCC) and grown in a minimal essential medium (Invitrogen), supplemented with 5% heat-inactivated fetal calf serum in 5% CO 2 at 37°C.

Techniques: In Vitro, Staining, Quantitation Assay

Journal: Advanced Pharmaceutical Bulletin

Article Title: The Role of Estrogen in Brain MicroRNAs Regulation

doi: 10.34172/apb.39216

Figure Lengend Snippet: The most abundant miRNAs in the brain

Article Snippet: miRNA-125 , miR-125b Overexpression led to an enhancement of neuronal proliferation and differentiation in neural stem/progenitor cells. miR-125a Increased neuronal differentiation levels induced by retinoic acid. , Neural tissue samples from the hippocampus of newborn Sprague–Dawley rat Mouse P19 embryonal carcinoma cells.

Techniques: Functional Assay, Inhibition, Expressing, Over Expression, Cell Culture, Transgenic Assay, Migration